Cross-talk between freezing response and signaling for regulatory transcriptions of MIR475b and its targets by miR475b promoter in Populus suaveolens.
Identifieur interne : 001936 ( Main/Exploration ); précédent : 001935; suivant : 001937Cross-talk between freezing response and signaling for regulatory transcriptions of MIR475b and its targets by miR475b promoter in Populus suaveolens.
Auteurs : Jun Niu [République populaire de Chine] ; Jia Wang [République populaire de Chine] ; Huiwen Hu [République populaire de Chine] ; Yinlei Chen [République populaire de Chine] ; Jiyong An [République populaire de Chine] ; Jian Cai [République populaire de Chine] ; Runze Sun [République populaire de Chine] ; Zhongting Sheng [République populaire de Chine] ; Xieping Liu [République populaire de Chine] ; Shanzhi Lin [République populaire de Chine]Source :
- Scientific reports [ 2045-2322 ] ; 2016.
Descripteurs français
- KwdFr :
- Acide salicylique (pharmacologie), Acétates (pharmacologie), Congélation (MeSH), Cyclopentanes (pharmacologie), Données de séquences moléculaires (MeSH), Facteur de croissance végétal (pharmacologie), Oxylipines (pharmacologie), Populus (génétique), Populus (métabolisme), Régions promotrices (génétique) (MeSH), Séquence nucléotidique (MeSH), Transcriptome (effets des médicaments et des substances chimiques), Transduction du signal (effets des médicaments et des substances chimiques), Transduction du signal (génétique), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme), microARN (génétique), microARN (métabolisme), Éléments de régulation transcriptionnelle (génétique).
- MESH :
- effets des médicaments et des substances chimiques : Transcriptome, Transduction du signal.
- génétique : Populus, Transduction du signal, Végétaux génétiquement modifiés, microARN, Éléments de régulation transcriptionnelle.
- métabolisme : Populus, Végétaux génétiquement modifiés, microARN.
- pharmacologie : Acide salicylique, Acétates, Cyclopentanes, Facteur de croissance végétal, Oxylipines.
- Congélation, Données de séquences moléculaires, Régions promotrices (génétique), Séquence nucléotidique.
English descriptors
- KwdEn :
- Acetates (pharmacology), Base Sequence (MeSH), Cyclopentanes (pharmacology), Freezing (MeSH), MicroRNAs (genetics), MicroRNAs (metabolism), Molecular Sequence Data (MeSH), Oxylipins (pharmacology), Plant Growth Regulators (pharmacology), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Populus (genetics), Populus (metabolism), Promoter Regions, Genetic (MeSH), Regulatory Elements, Transcriptional (genetics), Salicylic Acid (pharmacology), Signal Transduction (drug effects), Signal Transduction (genetics), Transcriptome (drug effects).
- MESH :
- chemical , genetics : MicroRNAs.
- chemical , metabolism : MicroRNAs.
- chemical , pharmacology : Acetates, Cyclopentanes, Oxylipins, Plant Growth Regulators, Salicylic Acid.
- drug effects : Signal Transduction, Transcriptome.
- genetics : Plants, Genetically Modified, Populus, Regulatory Elements, Transcriptional, Signal Transduction.
- metabolism : Plants, Genetically Modified, Populus.
- Base Sequence, Freezing, Molecular Sequence Data, Promoter Regions, Genetic.
Abstract
MicroRNAs (miRNAs) are small, non-coding RNAs that play important roles in post-transcriptional regulation of their target genes, yet the transcriptional regulation of plant miRNAs by promoter is poorly understood. Here, we firstly clone pri-miR475b cDNA and its native promoter from P. suaveolens, and characterize Psu-MIR475b as class-II gene transcribed by RNA polymerase II. By 5' deletion analysis of Psu-miR475b promoter in a series of promoter-GUS chimeric vectors, we functionally identify three positive regulatory regions and multiple cis-acting elements responsible for Psu-miR475b promoter activity in response to freezing stress and exogenous hormone treatment. Moreover, the Psu-miR475b promoter activity displays a tissue-specific manner, negatively regulated by freezing stress and positively by MeJA, SA or GA treatment. Importantly, we comparatively analyze the time-course transcriptional profiles of Psu-miR475b and its targets in Psu-miR475b over-expression transgenic plants controlled by Psu-miR475b-specific promoter or CaMV 35S constitutive promoter, and explore the regulatory mechanism of Psu-miR475b promoter controlling transcriptional expressions of Psu-MIR475b and its targets in response to freezing stress and exogenous hormone treatment. Our results reveal that Psu-miR475b promoter-mediated transcriptions of Psu-MIR475b and its targets in response to freezing stress may be involved in a cross-talk between freezing response and stress signaling process.
DOI: 10.1038/srep20648
PubMed: 26853706
PubMed Central: PMC4745078
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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wicri:level="1"><nlm:affiliation>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Jia" sort="Wang, Jia" uniqKey="Wang J" first="Jia" last="Wang">Jia Wang</name><affiliation wicri:level="1"><nlm:affiliation>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Hu, Huiwen" sort="Hu, Huiwen" uniqKey="Hu H" first="Huiwen" last="Hu">Huiwen Hu</name><affiliation wicri:level="1"><nlm:affiliation>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Chen, Yinlei" sort="Chen, Yinlei" uniqKey="Chen Y" first="Yinlei" last="Chen">Yinlei Chen</name><affiliation wicri:level="1"><nlm:affiliation>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="An, Jiyong" sort="An, Jiyong" uniqKey="An J" first="Jiyong" last="An">Jiyong An</name><affiliation wicri:level="1"><nlm:affiliation>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Cai, Jian" sort="Cai, Jian" uniqKey="Cai J" first="Jian" last="Cai">Jian Cai</name><affiliation wicri:level="1"><nlm:affiliation>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Sun, Runze" sort="Sun, Runze" uniqKey="Sun R" first="Runze" last="Sun">Runze Sun</name><affiliation wicri:level="1"><nlm:affiliation>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Sheng, Zhongting" sort="Sheng, Zhongting" uniqKey="Sheng Z" first="Zhongting" last="Sheng">Zhongting Sheng</name><affiliation wicri:level="1"><nlm:affiliation>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Liu, Xieping" sort="Liu, Xieping" uniqKey="Liu X" first="Xieping" last="Liu">Xieping Liu</name><affiliation wicri:level="1"><nlm:affiliation>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Lin, Shanzhi" sort="Lin, Shanzhi" uniqKey="Lin S" first="Shanzhi" last="Lin">Shanzhi Lin</name><affiliation wicri:level="1"><nlm:affiliation>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acetates (pharmacology)</term><term>Base Sequence (MeSH)</term><term>Cyclopentanes (pharmacology)</term><term>Freezing (MeSH)</term><term>MicroRNAs (genetics)</term><term>MicroRNAs (metabolism)</term><term>Molecular Sequence Data (MeSH)</term><term>Oxylipins (pharmacology)</term><term>Plant Growth Regulators (pharmacology)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Promoter Regions, Genetic (MeSH)</term><term>Regulatory Elements, Transcriptional (genetics)</term><term>Salicylic Acid (pharmacology)</term><term>Signal Transduction (drug effects)</term><term>Signal Transduction (genetics)</term><term>Transcriptome (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acide salicylique (pharmacologie)</term><term>Acétates (pharmacologie)</term><term>Congélation (MeSH)</term><term>Cyclopentanes (pharmacologie)</term><term>Données de séquences moléculaires (MeSH)</term><term>Facteur de croissance végétal (pharmacologie)</term><term>Oxylipines (pharmacologie)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Régions promotrices (génétique) (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Transcriptome (effets des médicaments et des substances chimiques)</term><term>Transduction du signal (effets des médicaments et des substances chimiques)</term><term>Transduction du signal (génétique)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (métabolisme)</term><term>microARN (génétique)</term><term>microARN (métabolisme)</term><term>Éléments de régulation transcriptionnelle (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>MicroRNAs</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>MicroRNAs</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Acetates</term><term>Cyclopentanes</term><term>Oxylipins</term><term>Plant Growth Regulators</term><term>Salicylic Acid</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Signal Transduction</term><term>Transcriptome</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Transcriptome</term><term>Transduction du signal</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term><term>Regulatory Elements, Transcriptional</term><term>Signal Transduction</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Populus</term><term>Transduction du signal</term><term>Végétaux génétiquement modifiés</term><term>microARN</term><term>Éléments de régulation transcriptionnelle</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Populus</term><term>Végétaux génétiquement modifiés</term><term>microARN</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Acide salicylique</term><term>Acétates</term><term>Cyclopentanes</term><term>Facteur de croissance végétal</term><term>Oxylipines</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Freezing</term><term>Molecular Sequence Data</term><term>Promoter Regions, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Congélation</term><term>Données de séquences moléculaires</term><term>Régions promotrices (génétique)</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">MicroRNAs (miRNAs) are small, non-coding RNAs that play important roles in post-transcriptional regulation of their target genes, yet the transcriptional regulation of plant miRNAs by promoter is poorly understood. Here, we firstly clone pri-miR475b cDNA and its native promoter from P. suaveolens, and characterize Psu-MIR475b as class-II gene transcribed by RNA polymerase II. By 5' deletion analysis of Psu-miR475b promoter in a series of promoter-GUS chimeric vectors, we functionally identify three positive regulatory regions and multiple cis-acting elements responsible for Psu-miR475b promoter activity in response to freezing stress and exogenous hormone treatment. Moreover, the Psu-miR475b promoter activity displays a tissue-specific manner, negatively regulated by freezing stress and positively by MeJA, SA or GA treatment. Importantly, we comparatively analyze the time-course transcriptional profiles of Psu-miR475b and its targets in Psu-miR475b over-expression transgenic plants controlled by Psu-miR475b-specific promoter or CaMV 35S constitutive promoter, and explore the regulatory mechanism of Psu-miR475b promoter controlling transcriptional expressions of Psu-MIR475b and its targets in response to freezing stress and exogenous hormone treatment. Our results reveal that Psu-miR475b promoter-mediated transcriptions of Psu-MIR475b and its targets in response to freezing stress may be involved in a cross-talk between freezing response and stress signaling process. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26853706</PMID><DateCompleted><Year>2017</Year><Month>01</Month><Day>09</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>6</Volume><PubDate><Year>2016</Year><Month>Feb</Month><Day>08</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>Cross-talk between freezing response and signaling for regulatory transcriptions of MIR475b and its targets by miR475b promoter in Populus suaveolens.</ArticleTitle><Pagination><MedlinePgn>20648</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/srep20648</ELocationID><Abstract><AbstractText>MicroRNAs (miRNAs) are small, non-coding RNAs that play important roles in post-transcriptional regulation of their target genes, yet the transcriptional regulation of plant miRNAs by promoter is poorly understood. Here, we firstly clone pri-miR475b cDNA and its native promoter from P. suaveolens, and characterize Psu-MIR475b as class-II gene transcribed by RNA polymerase II. By 5' deletion analysis of Psu-miR475b promoter in a series of promoter-GUS chimeric vectors, we functionally identify three positive regulatory regions and multiple cis-acting elements responsible for Psu-miR475b promoter activity in response to freezing stress and exogenous hormone treatment. Moreover, the Psu-miR475b promoter activity displays a tissue-specific manner, negatively regulated by freezing stress and positively by MeJA, SA or GA treatment. Importantly, we comparatively analyze the time-course transcriptional profiles of Psu-miR475b and its targets in Psu-miR475b over-expression transgenic plants controlled by Psu-miR475b-specific promoter or CaMV 35S constitutive promoter, and explore the regulatory mechanism of Psu-miR475b promoter controlling transcriptional expressions of Psu-MIR475b and its targets in response to freezing stress and exogenous hormone treatment. Our results reveal that Psu-miR475b promoter-mediated transcriptions of Psu-MIR475b and its targets in response to freezing stress may be involved in a cross-talk between freezing response and stress signaling process. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Niu</LastName><ForeName>Jun</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Jia</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Huiwen</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Yinlei</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>An</LastName><ForeName>Jiyong</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cai</LastName><ForeName>Jian</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Runze</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sheng</LastName><ForeName>Zhongting</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Xieping</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Shanzhi</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>02</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Sci Rep</MedlineTA><NlmUniqueID>101563288</NlmUniqueID><ISSNLinking>2045-2322</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000085">Acetates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003517">Cyclopentanes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D035683">MicroRNAs</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D054883">Oxylipins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010937">Plant Growth Regulators</NameOfSubstance></Chemical><Chemical><RegistryNumber>900N171A0F</RegistryNumber><NameOfSubstance UI="C072239">methyl jasmonate</NameOfSubstance></Chemical><Chemical><RegistryNumber>O414PZ4LPZ</RegistryNumber><NameOfSubstance UI="D020156">Salicylic Acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000085" MajorTopicYN="N">Acetates</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003517" MajorTopicYN="N">Cyclopentanes</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005615" MajorTopicYN="Y">Freezing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D035683" MajorTopicYN="N">MicroRNAs</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D054883" MajorTopicYN="N">Oxylipins</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010937" MajorTopicYN="N">Plant Growth Regulators</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D050436" MajorTopicYN="N">Regulatory Elements, Transcriptional</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020156" MajorTopicYN="N">Salicylic Acid</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059467" MajorTopicYN="N">Transcriptome</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>06</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>01</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>2</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>2</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>1</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26853706</ArticleId><ArticleId IdType="pii">srep20648</ArticleId><ArticleId IdType="doi">10.1038/srep20648</ArticleId><ArticleId IdType="pmc">PMC4745078</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>PLoS Genet. 2014 Apr;10(4):e1004266</Citation><ArticleIdList><ArticleId IdType="pubmed">24699192</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Plant Biol. 2014;14:271</Citation><ArticleIdList><ArticleId IdType="pubmed">25330732</ArticleId></ArticleIdList></Reference><Reference><Citation>RNA. 2006 Sep;12(9):1612-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16888323</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 2004 Oct 13;23(20):4051-60</Citation><ArticleIdList><ArticleId IdType="pubmed">15372072</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2008 Jul;55(1):131-51</Citation><ArticleIdList><ArticleId IdType="pubmed">18363789</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2002 Sep 20;297(5589):2053-6</Citation><ArticleIdList><ArticleId IdType="pubmed">12242443</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2014;9(4):e95800</Citation><ArticleIdList><ArticleId IdType="pubmed">24759739</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol Biochem. 2013 May;66:20-5</Citation><ArticleIdList><ArticleId IdType="pubmed">23454294</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2008 May 30;320(5880):1185-90</Citation><ArticleIdList><ArticleId IdType="pubmed">18483398</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2009 Feb 20;136(4):669-87</Citation><ArticleIdList><ArticleId IdType="pubmed">19239888</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 2012 Jul 30;586(16):2332-7</Citation><ArticleIdList><ArticleId IdType="pubmed">22659182</ArticleId></ArticleIdList></Reference><Reference><Citation>RNA. 2008 May;14(5):836-43</Citation><ArticleIdList><ArticleId IdType="pubmed">18356539</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol Biochem. 2012 Feb;51:116-22</Citation><ArticleIdList><ArticleId IdType="pubmed">22153247</ArticleId></ArticleIdList></Reference><Reference><Citation>Gene. 2010 Jul 1;459(1-2):39-47</Citation><ArticleIdList><ArticleId IdType="pubmed">20350593</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2005 Nov;17(11):3155-75</Citation><ArticleIdList><ArticleId IdType="pubmed">16214899</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2015 Mar;81(6):861-70</Citation><ArticleIdList><ArticleId IdType="pubmed">25619693</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:571-599</Citation><ArticleIdList><ArticleId IdType="pubmed">15012220</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2015 Apr;82(2):193-207</Citation><ArticleIdList><ArticleId IdType="pubmed">25736223</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2008 Apr 4;133(1):128-41</Citation><ArticleIdList><ArticleId IdType="pubmed">18342362</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Environ. 2013 Dec;36(12):2207-18</Citation><ArticleIdList><ArticleId IdType="pubmed">23651319</ArticleId></ArticleIdList></Reference><Reference><Citation>Genomics. 2013 Mar;101(3):187-94</Citation><ArticleIdList><ArticleId IdType="pubmed">23295247</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Comput Biol. 2007 Mar 9;3(3):e37</Citation><ArticleIdList><ArticleId IdType="pubmed">17352530</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2006 Aug;141(4):1219-32</Citation><ArticleIdList><ArticleId IdType="pubmed">16896234</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Biol. 2006;57:19-53</Citation><ArticleIdList><ArticleId IdType="pubmed">16669754</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2002 Aug;14(8):1675-90</Citation><ArticleIdList><ArticleId IdType="pubmed">12172015</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Biol. 2007;8(6):R96</Citation><ArticleIdList><ArticleId IdType="pubmed">17543110</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Mol Biol. 2010;639:39-55</Citation><ArticleIdList><ArticleId IdType="pubmed">20387039</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Biol. 2006;57:781-803</Citation><ArticleIdList><ArticleId IdType="pubmed">16669782</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2012 Jun;159(2):721-38</Citation><ArticleIdList><ArticleId IdType="pubmed">22508932</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1987 Dec 20;6(13):3901-7</Citation><ArticleIdList><ArticleId IdType="pubmed">3327686</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Plant Sci. 2007 Oct;12(10):444-51</Citation><ArticleIdList><ArticleId IdType="pubmed">17855156</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2004 Jan 23;116(2):281-97</Citation><ArticleIdList><ArticleId IdType="pubmed">14744438</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2004 Aug;16(8):2001-19</Citation><ArticleIdList><ArticleId IdType="pubmed">15258262</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 2008 Nov;1779(11):780-8</Citation><ArticleIdList><ArticleId IdType="pubmed">18471443</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2014 Feb;26(2):741-53</Citation><ArticleIdList><ArticleId IdType="pubmed">24510721</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Genet. 2005 Aug;1(2):e26</Citation><ArticleIdList><ArticleId IdType="pubmed">16121258</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2005 Aug;138(4):2145-54</Citation><ArticleIdList><ArticleId IdType="pubmed">16040653</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2009;10:449</Citation><ArticleIdList><ArticleId IdType="pubmed">19772667</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2005 Aug;17(8):2186-203</Citation><ArticleIdList><ArticleId IdType="pubmed">15994906</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2010 May;62(3):454-62</Citation><ArticleIdList><ArticleId IdType="pubmed">20128885</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Biochem. 1976 May 7;72:248-54</Citation><ArticleIdList><ArticleId IdType="pubmed">942051</ArticleId></ArticleIdList></Reference><Reference><Citation>Gene. 2009 Feb 15;431(1-2):61-6</Citation><ArticleIdList><ArticleId IdType="pubmed">19073239</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2008 Jul 22;105(29):9851-2</Citation><ArticleIdList><ArticleId IdType="pubmed">18632572</ArticleId></ArticleIdList></Reference><Reference><Citation>Genes Dev. 2004 May 15;18(10):1187-97</Citation><ArticleIdList><ArticleId IdType="pubmed">15131082</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2009 Jul;70(4):385-401</Citation><ArticleIdList><ArticleId IdType="pubmed">19353277</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell. 2013 Nov 21;52(4):591-601</Citation><ArticleIdList><ArticleId IdType="pubmed">24267452</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2012 May;70(3):421-31</Citation><ArticleIdList><ArticleId IdType="pubmed">22247970</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Biol Evol. 2012;4(3):230-9</Citation><ArticleIdList><ArticleId IdType="pubmed">22223755</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2008 May;67(1-2):169-81</Citation><ArticleIdList><ArticleId IdType="pubmed">18273684</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 2012 Jan 13;417(2):892-6</Citation><ArticleIdList><ArticleId IdType="pubmed">22209794</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Niu, Jun" sort="Niu, Jun" uniqKey="Niu J" first="Jun" last="Niu">Jun Niu</name></noRegion><name sortKey="An, Jiyong" sort="An, Jiyong" uniqKey="An J" first="Jiyong" last="An">Jiyong An</name><name sortKey="Cai, Jian" sort="Cai, Jian" uniqKey="Cai J" first="Jian" last="Cai">Jian Cai</name><name sortKey="Chen, Yinlei" sort="Chen, Yinlei" uniqKey="Chen Y" first="Yinlei" last="Chen">Yinlei Chen</name><name sortKey="Hu, Huiwen" sort="Hu, Huiwen" uniqKey="Hu H" first="Huiwen" last="Hu">Huiwen Hu</name><name sortKey="Lin, Shanzhi" sort="Lin, Shanzhi" uniqKey="Lin S" first="Shanzhi" last="Lin">Shanzhi Lin</name><name sortKey="Liu, Xieping" sort="Liu, Xieping" uniqKey="Liu X" first="Xieping" last="Liu">Xieping Liu</name><name sortKey="Sheng, Zhongting" sort="Sheng, Zhongting" uniqKey="Sheng Z" first="Zhongting" last="Sheng">Zhongting Sheng</name><name sortKey="Sun, Runze" sort="Sun, Runze" uniqKey="Sun R" first="Runze" last="Sun">Runze Sun</name><name sortKey="Wang, Jia" sort="Wang, Jia" uniqKey="Wang J" first="Jia" last="Wang">Jia Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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